Increase in yield of mulberry protoplasts by treatment with chemical substance

Abstract

By improving the conventional enzyme solutions for the isolation of protoplasts, the increase in the yield of protoplasts was studied, and the following results were obtained. To the enzyme solution for protoplast isolation which was prepared according to Saito's report (partly modified), 2, 4-Dichlorophenoxy acetic acid (2, 4-D) and Benzyladenine (BA) were added to increase the yield of the protoplasts. When mulberry leaves were used, the yield increased upon the addition of 2, 4-D at a concentration of 0.02ppm and BA at a concentration of 0.20ppm respectively. Furthermore, when the mulberry callus was used, the yield increased upon the addition of 2, 4-D at a concentration of 0.20ppm. When protoplasts were isolated from a mulberry callus after subcultures, the yield decreased compared with that in the primary culture. However, the yield of the protoplasts isolated from a callus subcultured after addition of Dithiothreitol (DTT) to the medium, increased to a much lower degree than that of the protoplasts in the primary culture.