Abstract
Hypocotyl of mulberry aseptically obtained was subjected to static culture in a medium basically composed of LS medium, as follows the molar ratio of NO3- and NH4+ was modified to 5:1 and 10-4M 2.4-D as well as 2% glucose was added and the pH was adjusted to 5.6 (prior to high pressure steam sterilization), at 28°C under dark conditions for 20 days, leading to the successful separation of a large amount of cells from the tissues, within a very short period of time.
