Abstract
This study concerns the research conducted on the biocompatibility of medical synthetic polymers utilized in the field of maxillo-facial surgery, such as Realastic®, Epithane-3®, HC-107/ON-105 system and Silastic® MDX4-4210.
Three methods were used to evaluate the cytotoxicity to the L cell on each of these four polymers to investigate their biocompatibility. These three methods were 1) cell nucleus count, 2) morphological observation using a phase contrast microscope and 3) investigation of DNA, RNA and protein synthesis by the incorportion rate of radioactivity using 3H-thymidine, 3H-Uridine and 3H-Leucine.
Results:
Polmyer Cell nucleus count Morphology DNA, RNA and protein synthesis
REALASTIC® Cytostatic in 1-10mg/ ml
Cytostatic in 1-3.2mg/ml Cytocidal in 10mg/ml
DNA, RNA and protein inhibited but RNA inhibition was moderate.
EPITHANE-3®
Cytostatic in 1mg/ml Cytocidal in 3.2-10mg/ml
Cytostatic in 1mg/ml Cytocidal in 3.2-10mg/ml
DNA, RNA and protein inhibited.
HC-107/ON-105 system
Cytostatic in 10-100mg/ml
Cytostatic in10-100mg/ml
DNA, RNA and protein inhibited.
SILASTIC® MDX4-4210
Non-cytotoxicity in 10-100mg/ml
Non-cytotoxici- DNA and RNA inhibited moderatety in 10-100mg ly but protein accelerated./ml
Elution Tendency;
By repeated extraction five times during 24 hours using a medium containing 10% serum, the decrease in the DNA synthesis inhibition of the L cell was measured and the following results were obtained: Realastic®; 100% Epithane-3®; 860, HC-107/ON-105 system; 36% and Silastic® MDX4-4210; 76% .
