Investigation on the Conditions for Effective Detection of Melon necrotic spot virus from Infected Soils

Abstract

For detection of melon necrotic spot virus (MNSV) from infected soils, enzyme-linked immunosorbent assay (DAS-ELISA) has been applied to root samples of melon seedlings, pot-cultivated in the soil for three weeks in a growth chamber at 25°C in 16-h light / 8-h dark conditions. In order to increase the detection accuracy, it was shown that the incubation of sample seedlings should be carried out at 18°C or 20°C. Moreover, MNSV was detected by DAS-ELISA from all samples incubated for two weeks at changing temperature conditions (25°C or 30°C in light and 15°C in dark) showing that the test period was shortened by one week compared to plants incubated at a constant temperature condition (25°C in light and dark).