DNA Barcoding Based on Mitochondrial COI Gene of Hymenopterous Parasitoid Wasps on the Garden Pea Leafminer Fly, Chromatomyia horticola Collected in Japan

Abstract

Mitochondrial DNA sequences coding cytochrome c oxidase subunit I (COI) gene of 14 hymenopterous parasitoid wasps on the garden pea leafminer fly, Chromatomyia horticola were investigated. Dacnusa nipponica, Opius sp., Halticoptera circulus, Sphegigaster hamugurivora, Chrysocharis pentheus, Chrysocharis pubicornis, Closterocerus trifasciatus, Diglyphus albiscapus, Diglyphus isaea, Neochrysocharis formosa, Pediobius metallicus and Pnigalio katonis have different DNA sequences and can be discriminated among each other. Diglyphus minoeus and Merismus sp. were failed to determine the sequences.