Abstract
Specimens of Torymus beneficus (Yasumatsu et Kamijo), the indigenous and principal parasitoid of the chestnut gall wasp Doryocosmus kuriphilus (Yasumatsu), were collected from two sites in Nagano Prefecture, and were identified as to emergence strain (early- or late-season strain) using molecular markers: malic enzyme (ME), internal transcribed spacer 2 of nuclear ribosomal RNA gene (ITS2), and/or mitochondrial cytochrome oxidase subunit I (COI). The results indicated that the emergence periods of the two emergence strains of T. beneficus, early- and late-season strain, which were identified by genetic indices, can overlap, although the two strains were so far considered to have significantly different emergence periods.
