2019 Volume 10 Pages 71-74
At the mouth of Kumagawa River in Yatsushiro, green laver has been commercially cultivated as flavouring food. Although Shimantogawa's green laver was most famous in Japan and identified as Ulva prolifera, Kumagawa's green laver was not elucidated whether U. prolifera or other species. In this work, we attempt to identify green laver species grown in Kumagawa river using genetic marker (chloroplastic rbcL gene). A novel method for readily extract chloroplastic DNA from small species of leaf sample has been developed. From both live and dry samples, extraction of chloroplastic DNA were possible. The rbcL gene fragments were amplified by PCR using universal primer set, and directly sequenced. 1,119bp length of rbcL sequence was searched with BLAST, it was fully identical to rbcL sequences of U. linza, U. procera, and U. prolifera (LPP clade). However, Kumagawa's sample was considered as U. prolifera by its morphologically aspects, further analysises using other genetic markers (e.g. nuclear-encoded ITS and associated 5.8S rDNA regions) were needed.
