Abstract
In order to make muscles transparent after skeletal staining, all small bones are preserved in the transparent skeletal specimen without destroying the three-dimensional structure. In particular, small fish can observe the skeleton of the whole body, so they are suitable for learning the role of bones. Currently, it is possible to obtain transparent skeletal specimens and specimen preparation kits from science teaching materials companies, and it can be seen that their use in classes is expanding. However, transparent skeletal specimens are too expensive to prepare for each student. It is important to remove of protein and lipid for clearing specimens. In this practice, we tried to clear specimen of medaka by immersing in dish detergent for a long time, and found that it became so good transparent that the vertebrae could be observed. In addition, in this practice, each student was asked to work on different experimental conditions, and the results were evaluated and considered by each other. From this practical process, we propose collaborative inquiry learning in which a large number of hypothesis settings are shared by students or groups, and they are brought together to draw conclusions.
