Abstract
Passive haemagglutination test was attempted to apply for the screening of human-leukocyte-antigen (HLA) antibodies in human sera, in comparison with the micro-cytotoxicity test. Lymphocytes and granulocytes from group O human bloods were separated by the specific gravity centrifugation, and classified according to the NIH lymphocyte cytotoxicity (LC) test ; both cells were then sonicated into antigen solutions and adsorbed onto formol-alcohol-treated tanned sheep red cells. Sera with positive LC tests agglutinated the antigen-coated tanned cells at 1 : 8-64 dilutions ; antibody negative sera against LC tests gave no agglutinations. The results indicate the correlation between the LC tests and the passive haemagglutination tests.
