Abstract
To evaluate the lymphocyte subsets with the advance of the cancer stage in the whole blood of the 70 patients with carcinoma (27 of esophagus, 24 of stomach, 7 of large intestine, 1 of liver, 1 of choledochus, 10 of breast) and 8 patients with benign disease were investigated by the application of a rapid flowcytometric immunofluorescence method and by use of monoclonal antibodies OKT3, OKT4, OKT8 and OKIa1, and T-lymphocyte ratio, B-lymphocyte ratio and response of lymphocyte to ConA, PHA were also examined at the same time. The results were as follows : 1) OKT3^+ and OKIa1^+-lymphocyte ratio did not show a significant difference in advancement of the cancer stage, but OKT4^+-lymphocyte ratio was decreased and OKT8^+lymphocyte ratio was increased in advancing the cancer stage with significant difference (p<0.01). 2) There was not a significant correlation between ConA/PHA and OKT4^+/OKT8^+ (r=2108). 3) There were significant correlations between OKT3^+, OKT4^+, OKT8^+, OKIa1lymphocyte count and absolute lymphocyte count (r=0.9482, 0.8231, 0.7822, 0.5481). 4) There was not a significant correlation between OKT3^+-lymphocyte ratio and T-lymphocyte ratio (r=0.3820, T=1.754). 5) There was not a significant correlation between OKIa1^+-lymphocyte ratio and B-lymphocyte ratio (r=0.3158).
