1986 Volume 17 Issue 4 Pages 477-488
It has been established that there is glucose intolerance in the patients with obstructive jaundice. However, the interaction of insulin and glucagon receptors of hepatic plasma membranes in rats with obstructive jaundice has not been studied. Mechanical cholestasis in rats was induced by double ligation and excision of the common bile duct. Sham-operated rats were referred to as their controls. After 7 days, plasma membranes of rat liver were prepared according to the method of Lesko. As marker enzyme for the plasma membrane, Na^+, K^+-ATPase activity was assayed. The bindings of insulin and glucagon to liver plasma membranes were measured. Specific bindings of ^<125>I-insulin and ^<125>I-glucagon with hepatic plasma membrane in rats with bile duct excision decreased. Kinetic studies by the Scatchard plot analysis of these results revealed decrease in insulin binding affinity and number of glucagon receptors. Activities of Na^+, K^+-ATPase also decreased in bile duct excised rats. These results suggest that alterations in receptors of insulin and glucagon are involved in mechanisms of glucose intolerance in human obstructive jaundice.
