Effect of CMPF – 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid- as an oxidation accelerator

Abstract

We examined the enhanced effect of Fenton reaction by furan fatty acid metabolite, 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF) . It is known that CMPF may induce uremia and diabetes, however, its role is not known so far. To evaluate the enhanced effect of Fenton reaction (Fe^2＋＋H₂O₂→･OH＋Fe^3＋＋OH⁻) by CMPF, the breaks of plasmid DNA has been measured using a electrophoresis. It was found that the plasmid DNA was broken by reactive oxygen species -hydroxyl radical (･OH) - accelerated by CMPF. In addition, electron spin resonance shows that CMPF accelerated the ･OH formation from Fenton reaction by reducing Fe^3＋ to Fe^2＋. Furthermore, quantum chemical calculation shows clearly that CMPF forms iron ions to stabilize the chelate complex, however, it does not form copper ion complex. These results suggest that oxidative degradation may be induced by CMPF.