2016 Volume 62 Pages 34-42
Glycine soja Sieb. et Zucc., an ancestral wild species of soybean （G. max （L.） Merr.） has been gathering attention as a genetic resource. In addition, considering the cultivation of genetically modified soybean will be introduced to Japan in the future, crossing species might affect biological diversity. Under such a situation, bacterial diseases of G. soja have not yet been reported so far. Therefore, pathogenic bacteria of G. soja and its characteristics were investigated in this study. Occurrence of bacterial disease of G. soja in various parts of Japan was surveyed and 90 bacterial isolates were isolated from the infected leaves. Based on the tobacco hypersensitive reaction（ HR） or hypersensitive like reaction（ HLR）, 17 and 35 isolates of presumably plant pathogenic bacteria were isolated. By homology analysis based on 16S rDNA sequences, 52 isolates comprising 13 genera such as Xanthomonas spp., Pseudomonas spp., Rhizobium sp. and Pantoea sp were identified. After spray inoculation to G. soja and G. max with those 52 isolates, 13 isolates were pathogenic to both plants and divided into isolates with two colony colors of yellow and white. Three white colony isolates had high homology to P. syringae pv. glycinea, whereas 10 yellow ones formed the same cluster with X. axonopodis pv. glycines.
