Abstract
Barley yellow dwarf virus (BYDV), occurring in Kyushu, was purified and the antiserum against BYDV was prepared. The antiserum reacted with BYDV up to a dilution of 1/160 in precipitin ring interface test. Using this antiserum, the serological diagnosis of BYDV by ELISA was developed. BYDV in the leaf extract of infected barley plants cpuld be detected up to a dilution of 1/640 by ELISA. Concentration of BYDV in barley plants showing yellow symptoms was higher in the upper to middle leaves than in the lower leaves. BYDV was frequently detected in barley and wheat plants showing yellow symptoms in the fields of Kyushu National Agricultural Eperiment Station.
