Abstract
Reversed phase high-performance liquid chromatography(HPLC)with an ODS porous glass column(HITACHI GEL#3161)was used to assay estrone, 17-β-estradiol, and estriol.Using acetonitrile-water(30/70)as a mobile phase at a flow rate of 1 ml/min, the three steroids were analysed in 5 min.The results demonstrate that the present packing materials was much advantageous to analyse these three steroids.
