Abstract
Laser speckle microscopy was developed to observe a single living cell without any troublesome labeling preparation. The system consists of an optical microscope, a laser source, and a video camera. The technique has an advantage that ellular conditions can be instantaneously determined by observing speckle fluctuations. In the experiments, living and fixed HeLa cells were compared on the basis of speckle fluctuation and frequency spectra. By applying this technique to cell observations, it is expected to estimate not only cellular activity but also real-time movements of intercellular materials including the cytoplasm without fluorescence observation.
