Abstract
We demonstrated observation of glycerinated muscle with near-field recording technique that we have developed. Using this technique, optical field distributions localized near specimens are recorded as surface topographic distributions of a photosensitive film. The topographic distribution is detected with a high resolution microscope such as AFM. The technical system is no requirement to scan the optical probe for illuminating the sample or detecting the light localized near the specimen. Furthermore, the system can observed moving or soft specimens. We show that striped periodic structures of muscle cell before and after muscle contraction and lengths of each inner structure of muscle cell such as A-band, I-band, and Z-line can be measured with wavelength resolution. Our technique can achieve higher contrast and the periodic structure of glycerinated muscles was clearly observed compared to conventional optical microscope image.
