Abstract
Observation of a living plant was performed by a laser speckle microscopy. The merit of this technique is that it is possible to easily obtain information of organisms without any special preparations, such as staining and gene transfection. In this study, a living leaf was observed for half a day at intervals of one hour. To our knowledge, this is the first report of a long-term tissue observation by the laser speckle. It was possible to visualize the fading process of the leaf cut off from the living plant. It was found that the temporal fluctuation of the speckles on the leaf completely ceased within 10 hours after cutting off the leaf from the plant body.
