Abstract
We applied Raman spectroscopy to monitor the molecular compositional changes during the maturation
of cultured neural cells. Since the Raman spectra of the nucleus and cytoplasm of neural cells are
slightly different, we employed the Raman spectra of the nucleus for further analysis. We analyzed with
PLS regression the spectra of live neural cells measured after 2, 8, 15, and 60 days of culturing. Our
results suggest that functional progress and maturation are related with molecular compositional changes
in the cell and demonstrate the high potential of Raman spectroscopy in monitoring functions of live
neural cells without labeling.
