Abstract
In this study, we present a method to determine the reduced scattering coefficients μs’ (λ ) and the
absorption coeffi cients μa (λ ) of in vivo cerebral cortex based on the diffuse refl ectance spectroscopy
using single reflectance fiber probe with two source-collector geometries. In order to confirm the
possibility of the method to evaluate changes in the optical properties of biological tissue, we performed
in vivo experiments for exposed brain of rats during cortical spreading depression (CSD). The time
courses of μa (λ ) and SO2 after the stimulation indicated the hemodynamic change in cerebral cortex,
whereas variations of μs’(λ ) implied the changes in light scattering by tissue and red blood cells.
