Abstract
Recent development in super resolution microscopy has enabled noninvasive observation of various
kinds of specimens with high spatial-resolution. Despite the potential of the chemical analysis in Raman
microscopy, high-resolution analytical imaging has been challenging due to the weak efficiency in
Raman scattering. We introduce structured illumination to spontaneous Raman microscopy in order to
realize a technique for large-area and high-spatial resolution micro-spectroscopic imaging. We
developed a structured line illumination Raman microscope using two beam interference in a line
illumination and demonstrated high-resolution Raman imaging of polymer nanoparticles and a biological
tissue. The results indicate that the high resolution Raman imaging would strongly enhance the
analytical capability in chemical imaging of medical, biological and industrial materials.
