High-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering MicroscopyHigh-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering MicroscopyHigh-Speed Label-Free Spectroscopic Biological Imaging Based on Stimulated Raman Scattering Microscopy

Abstract

Stimulated Raman scattering (SRS) microscopy is a powerful method for the high-speed imaging of biological samples without labeling or staining. This method is based on the sensitive detection of molecular vibrational signatures probed by two-color picosecond pulses. This review introduces the principle and applications of SRS microscopy and explains various methods of SRS spectral imaging for discriminating different constituents.