Abstract
Biofluorometric bio-sniffers (biochemical gas sensor) for acetone and isopropanol (IPA) were
constructed by connecting a UV-LED and a photomultiplier tube to an optical fiber with a flow-cell
equipped an S-ADH (secondary alcohol dehydrogenase) membrane. Gaseous acetone and IPA were
measured by detecting auto-fluorescence of nicotinamide adenine dinucleotide (NADH) (ex. 340 nm, fl.
491 nm) as a coenzyme of S-ADH redox reactions. Both breath volatiles from healthy and diabetic
subjects were analyzed by the bio-sniffers, thus diabetic acetone showing significant higher than that of
heathy subjects. Similarly, a volatile-imaging system (sniff-cam) employing alcohol dehydrogenase
(ADH) that detects acetaldehyde (AcH) vapor was also developed using a ADH mesh, a UV-LED array
sheet and high sensitive camera, and applied for imaging AcH in breath and skin gas after drinking. The
ADH sniff-cam allowed to visualize the concentration distribution of AcH in breath and skin gas. The
biofluorometric sniff-devices would be useful for non-invasive disease screening and assessment.
