The Review of Laser Engineering
Online ISSN : 1349-6603
Print ISSN : 0387-0200
ISSN-L : 0387-0200
Application of SHG Microscopy for Skin Measurements
Eiji HASETakeshi YASUI
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2020 Volume 48 Issue 12 Pages 665-


Second-harmonic-generation (SHG) microscopy is an attractive imaging modality for the in situ visualizations of collagen fiber in tissues. For example, collagen fibers in dermis can be visualized as the optical sectioning of 3D images with high selectivity, good image contrast, high spatial resolution, and moderate penetration without staining. Furthermore, the image analysis of SHG images enables us to obtain the quantitative parameters of collagen synthesis, assembly, and distribution; polarization analysis gives additional information in terms of microscopic fiber orientation. In this paper, we introduce our recent research progress and discuss the advantages of SHG microscopy in skin measurements.

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© 2020 by The Laser Society of Japan
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