2021 Volume 49 Issue 5 Pages 276-
Spectroscopic imaging unveils what kinds of biomolecules exist in cells, tissues, or animals. Coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging enables us to obtain molecular information in a living model organism, Caenorhabditis elegans. The microspectroscopic system employs a new laser source based on the dual-fiber-output configuration, which generates 50-ps laser pulses with 1 MHz repetition rate. The system enables us to conduct label-free spectroscopic imaging in vivo. Spectroscopic imaging has the potential to uncover crucial questions in life sciences.