Abstract
The N2 laser microbeam is irradiated on the nuclei of the callus cell of Tabacco leaf by varying the power density of the laser light in the range from 2.8×1012 to 2.7×1013 W/m2. It is found that the does action curve clearly depends on the peak power, indicating that two photon absorption process plays an important role in the leathal effect. The details of the design and the construction of the N2 laser microbeam system are also presented.
