Abstract
Localised surface plasmon field around nanowells on gold film has been applied to enhance the fluorescence of BODIPY-dyes and Eu (TTF) 3 towards ultra-high throughput fluorescence analysis using nanowells integrated on a microtip. A 100 nm gold film with nanowells have been prepared on a 0.17 mm glass cover-slip by projection method using 500 nm or 1μm polystyrene spheres, and then fluorescence-stained with BODIPY-dyes or Eu (TTF) 3. The gold films have been excited by grazing illumination or by ATR using a 404 nm semiconductor laser. Both excitation methods gave remarkably enhanced fluorescence at the nanowells. The fluorescence image by ATR demonstrates that the surface plasmon field is strongly localised and scattered at the nanoholes giving comet-like spots.
