Abstract
M. tuberculosis var. hominis, E. coli, S. typhi, P. tularensis, N. gonorrhoeae and Candida albicans were found to synthesize methionine from homocysteine and choline. M. tuberculosis var. hominis, E. coli, S. typhi and Candida albicans of formed glycocyamine from arginine and glycine, from which, by the further addition of methionine, creatine was produced. For the formation from pure glycocyamine and methionine, the coexistence of V. B12, was necessary. The synthesis of creatine by M. tuberculosis var. hominis was completely inhibited by 5×10-2M of INH, while SM, PAS, TB, CM, CTC, OTC and EM showed no inhibitory effect. With the other organisms tested, INH showed no inhibitory effect on the synthetic activity mentioned above. This inhibition caused by INH on M. tuberculosis var. hominis was antagonized by ATP, folic acid and V. B6. M. tuberculosis var. hominis produced sarcosine and urea from ereatine. This ability was not influenced by INH or other antibiotics tested.
Both P. tularensis and N. gonorrhoeae did not produced creatine from glyco-cyamine, arginine, glycine, or methionine.
