Immunochemical and Biochemical Studies of Fungi

XIII. On the Galactomannans Isolated from Mycelia and Culture Filtrates of Several Filamentous Fungi

Abstract

Polysaccharides were separated from mycelia and culture filtrates of the filamentous fungi Aspergillus niger and Penicillium chrysogenum, and purified by column chromato-graphy on Sephadex G-50, DEAE- and CM-cellulose, successively. No nitrogen and phos-phorus were detected in the polymer, and the sugar components were observed to be galactose and mannose. The polysaccharides were confirmed to be galactomannans which were easily hydrolyzed by weak acid, liberating galactofuranose and oligosugar in dialyza-ble fractions.