Use of a Diffusion Barrier Technique in Bioautography

Abstract

A diffusion barrier was used in bioautography to prevent the diffusion of compounds separated by chromatography or electrophoresis in a seeded agar toward the separation direction. Two main advantages were demonstrated in the present method; (1) better separation and identification of compounds occurring closely positioned, and (2) more sensitive detection owing to compact growth zones. The present method has proved satisfactory and reliable for the routine detection of unknown growth factors.