Replication and Transfer of the R Factor in a Synchronized Culture of Escherichia coli

Yoriko YABE; Susumu MITSUHASHI

doi:10.1111/j.1348-0421.1971.tb00547.x

Yoriko YABE, Susumu MITSUHASHI

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1971 Volume 15 Issue 1 Pages 21-27

DOI https://doi.org/10.1111/j.1348-0421.1971.tb00547.x

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Published: 1971 Received: August 24, 1970 Available on J-STAGE: April 18, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: April 18, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : According to mutation mapping after nitrosoguanidine treatment of successive samples obtained from a synchronized culture of Escherichia coli K12 carrying an R factor, it was found that the majority of mutations of the R factor were produced after a maximum peak of galactose mutant production. According to the order of the chromosome replication and the initiation point of the replication, these facts suggest that the replication of the R factor occurs after the replication of the galactose gene. The transfer frequency of the R factor from a synchronized donor culture to the nonsynchronized
recipient was the highest after the replication of the chlotamphenicol gene on the R factor, probably after the replication of the R factor genome. The recipient ability of the R factor in a synchronized recipient culture from a nonsynchronized donor was the highest after a maximum peak of the galactose mutation, and probably after the cell division in the recipient.
Right : According to mutation mapping after nitrosoguanidine treatment of successive samples obtained from a synchronized culture of Escherichia coli K12 carrying an R factor, it was found that the majority of mutations of the R factor were produced after a maximum peak of galactose mutant production. According to the order of the chromosome replication and the initiation point of the replication, these facts suggest that the replication of the R factor occurs after the replication of the galactose gene. The transfer frequency of the R factor from a synchronized donor culture to the nonsynchronized recipient was the highest after the replication of the chlotamphenicol gene on the R factor, probably after the replication of the R factor genome. The recipient ability of the R factor in a synchronized recipient culture from a nonsynchronized donor was the highest after a maximum peak of the galactose mutation, and probably after the cell division in the recipient.

Date of correction: April 18, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

According to mutation mapping after nitrosoguanidine treatment of successive samples obtained from a synchronized culture of Escherichia coli K12 carrying an R factor, it was found that the majority of mutations of the R factor were produced after a maximum peak of galactose mutant production. According to the order of the chromosome replication and the initiation point of the replication, these facts suggest that the replication of the R factor occurs after the replication of the galactose gene. The transfer frequency of the R factor from a synchronized donor culture to the nonsynchronized recipient was the highest after the replication of the chlotamphenicol gene on the R factor, probably after the replication of the R factor genome. The recipient ability of the R factor in a synchronized recipient culture from a nonsynchronized donor was the highest after a maximum peak of the galactose mutation, and probably after the cell division in the recipient.

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