Phosphorylated Inactivation of Aminoglycosidic Antibiotics by Pseudomonas aeruginosa

Abstract

The studies were conducted on the inactivation of aminoglycosidic antibiotics including a new antibiotic, lividomycin, by a cell-free fraction from a strain of Pseudomonas aeruginosa isolated from the urine of a patient with cystitis. The strain strongly inactivated No. 2230-C (mannosyl paromomycin), paromomycin, kanamycin, aminodeoxykanamycin and neomycin but did not inactivate lividomycin A, dihydrostreptomycin and gentamicin. Inactivated-No. 2230-C, -paromomycin and -kanamycin were isolated and purified using column chromatography techniques. Inactivated antibiotics were found to be reactivated by the treatment with alkaline phosphatase. According to the reactivation by alkaline phosphatase and analytical data, it was concluded that the inactivated products were monophosphorylated derivatives of each antibiotic.