Interferon-Inducing Activity of Acidic Polysaccharides II. Induction of Rabbit Serum Interferon by Chemically Phosphorylated Polysaccharides<sup>1</sup>

Shigeo SUZUKI; Masuko SUZUKI; Masanori IMAYA; Fumio CHAKI

doi:10.1111/j.1348-0421.1972.tb00620.x

Interferon-Inducing Activity of Acidic Polysaccharides

II. Induction of Rabbit Serum Interferon by Chemically Phosphorylated Polysaccharides¹

Shigeo SUZUKI, Masuko SUZUKI, Masanori IMAYA, Fumio CHAKI

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1972 Volume 16 Issue 1 Pages 1-5

DOI https://doi.org/10.1111/j.1348-0421.1972.tb00620.x

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Published: 1972 Received: June 14, 1971 Available on J-STAGE: April 18, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: April 18, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : Readily available polysaccharides, amylopectin, amylose, dextrin, and yeast mannan. were
chemically phosphorylated using polyphosphoric acid in the presence of a tertiary amine, and
the resultant phosphates were examined for their interferon-inducing activity in rabbits employ-
ing an assay system consisting of a primary culture of rabbit kidney cells and vesicular stomatitis
virus. All the phosphates were shown to be active as interferon inducer, and, especially, the
activity of those containing more than 2% phosphorus were quite strong. Interfnons evoked
by the above phosphates resembled those induced by bacterial endotoxin, e. g., the viral inhibiting
activity was susceptible to heat treatment, low pH and tryptic digestion. Since all the parent
polysaccharides showed no interferon-inducing activity. it is reasonable to assume that the activc
center of these inducers might reside or be due to the anionic phosphate groups.
Right : Readily available polysaccharides, amylopectin, amylose, dextrin, and yeast mannan. were chemically phosphorylated using polyphosphoric acid in the presence of a tertiary amine, and the resultant phosphates were examined for their interferon-inducing activity in rabbits employing an assay system consisting of a primary culture of rabbit kidney cells and vesicular stomatitis virus. All the phosphates were shown to be active as interferon inducer, and, especially, the activity of those containing more than 2% phosphorus were quite strong. Interfnons evoked by the above phosphates resembled those induced by bacterial endotoxin, e. g., the viral inhibiting activity was susceptible to heat treatment, low pH and tryptic digestion. Since all the parent polysaccharides showed no interferon-inducing activity. it is reasonable to assume that the activc center of these inducers might reside or be due to the anionic phosphate groups.

Date of correction: April 18, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

Readily available polysaccharides, amylopectin, amylose, dextrin, and yeast mannan. were chemically phosphorylated using polyphosphoric acid in the presence of a tertiary amine, and the resultant phosphates were examined for their interferon-inducing activity in rabbits employing an assay system consisting of a primary culture of rabbit kidney cells and vesicular stomatitis virus. All the phosphates were shown to be active as interferon inducer, and, especially, the activity of those containing more than 2% phosphorus were quite strong. Interfnons evoked by the above phosphates resembled those induced by bacterial endotoxin, e. g., the viral inhibiting activity was susceptible to heat treatment, low pH and tryptic digestion. Since all the parent polysaccharides showed no interferon-inducing activity. it is reasonable to assume that the activc center of these inducers might reside or be due to the anionic phosphate groups.

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