Slime Production by Pseudomonas aeruginosa

II. A New Synthetic Medium and Cultural Conditions Suitable for Slime Production by Pseudomonas aeruginosa

Abstract

Using Pseudomonas aeruginosa culture IFO 3445, the nutritional requirements and cultural conditions suitable for slime production were investigated. A synthetic medium was established from the experimental results, which was composed of sodium glutamate, glucose, phosphate and magnesium salt. When a cellophane plate method was used, incubation at 37C for 3 days attained the highest relative viscosity. In the presence of an oxidizable carbohydrate the relative viscosity of the culture fluid was reduced with the acidic reaction, and recovered if the reaction was adjusted to pH7-8.