Abstract
A surface antigen(s) was detected in adenovirus type 12 (Ad12)-transformed cells by fluorescent antibody technique. The antigen was revealed by antisera of mice and rabbits immunized with the virus, and by those of rabbits immunized with conditioned medium from cell cultures carrying the surface antigen. The antigen was specific for the Ad12-transformed cells, since it was found only in the Ad12-transformed cells regardless of the animal species and not in any other cells, including SV40-and polyoma virus-ransformed cells. The antigen was distinct from the Ad12-specific viral and T antigens, since the sera of rabbits immunized with the conditioned medium contained little or no detectable antibodies to the latter two antigens. On the contrary, the surface antigen was not detected in any tumor cells induced by Ad12 by the above antisera, and no detectable antibody to the surface antigen was produced in mice immunized with the tumor cells.
