Studies on the Growth Conditions of Campylobacter fetus

III. Collaborative Function of Some Surfactants in a Yeast Extract Medium for Selective Cultivation

Abstract

The collaborative function of some surfactants for selective cultivation of Campylobacter fetus was examined using a plain homemade yeast extract agar medium supplemented with thioglycollate as a simple basal medium. Out of the combinations of various growth-inhibiting substances with colistin used as the main selective growth-inhibiting surfactant, the combined use of colistin (250 u/ml) and either desoxycholate (0.01%) or taurocholate (0.02%) in addition to brilliant green (1:400000-500000) brought about a satisfactory result. There was no serious disorder of each selective inhibitory function for the growth of C. fetus and suppression to other bacteria, particularly to both Pseudomonas aeruginosa and Proteus vulgaris. It was presumed that this selectivity depended upon both the well-balanced combination in the quantities of the surfactants and the use of a plain basal medium such as the homemade yeast extract agar.