NUTRITION OF SALMONELLA PULLORUM AND SALMONELLA GALLINARUM I. GROWTH FACTOR AND AMINO ACID REQUIREMENTS

Abstract

Comparing 15 strains of Salmonella pullorum and 11 strains of Salmonella gallinarum in nutritional requirements the following results were obtained.
1. Three strains of S. pullorum and 6 strains of S. gallinarum did not require any growth factors, but of the other strains, a combination of 3 vitamins-niacin, thiamine and riboflavin-was necessary for growth.
2. By an elimination test a medium containing 7 amino acids-glycine, asparagine, glutamic acid, proline, cystine, arginine and tryptophan-was found to support a moderate growth of all tested strains.
3. S. pullorum is more exacting than S. gallinarum in both growth factor and amino acid requirement (proline, glutamic acid and cystine).