TWO REAGENTS FOR FLUORESCENT ANTIBODY TECHNIQUE AND THE VISUALIZATION OF THE VIRAL ANTIGEN SYNTHESIZED IN EHRLICH ASCITES TUMOR CELLS INFECTED WITH ED VIRUS (STUDIES ON VIRAL ONCOLYSIS 4.)

MICHIO ITO; KUSUYA NISHIOKA

doi:10.1111/j.1348-0421.1959.tb00103.x

MICHIO ITO, KUSUYA NISHIOKA

Author information

JOURNAL FREE ACCESS

1959 Volume 3 Issue 1 Pages 71-83

DOI https://doi.org/10.1111/j.1348-0421.1959.tb00103.x

Details

Published: 1959 Received: December 30, 1958 Available on J-STAGE: April 18, 2008 Accepted: - Advance online publication: - Revised: -
Correction information
Date of correction: April 18, 2008 Reason for correction: - Correction: TITLE Details: Wrong : TWO REAGENTS FOR FLUORESCENT ANTIBODY TECHNIQUE AND THE VISUALIZATION OF THE VIRAL ANTIGEN SYNTHESIZED IN EHRLICH ASCITES TUMOR CELLS INFECTED WITH ED VIRUS* (STUDIES ON VIRAL ONCOLYSIS 4.)
Right : TWO REAGENTS FOR FLUORESCENT ANTIBODY TECHNIQUE AND THE VISUALIZATION OF THE VIRAL ANTIGEN SYNTHESIZED IN EHRLICH ASCITES TUMOR CELLS INFECTED WITH ED VIRUS (STUDIES ON VIRAL ONCOLYSIS 4.)

Date of correction: April 18, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : 1) A fluorescent antibody technique using 1-dimethylaminonaphthalene 5-sul-phonylchloride and Xylene Red B has been described. The conjugation procedure
for each dye with protein is simple without any deterioration of the antibody activity.2) The quality of each conjugate was determined by use of a Beckman spectro-photometer and spectrofluorometer. 3) The viral antigen synthesized in Ehrlich ascites tumor cells infected with ED virus was distinctly visualized by staining of each conjugate, and the specificity of fluorescent staining of antigen was confirmed with the systematic control tests.
XRB-conjugated protein was preferable for the detection of antigen, because it showed brilliant orange fluorescence and could be clearly discriminated from the auto-fluorescene of cells. 4) The viral antigen was detected in the cytoplasm of the infected cells within 6 hous after virus challenge, and increased continuously until a maximum was reached at 24 hours. 5) The observation by mean of fluorescent antibody technique confirms the the previous results obtained by immunochemical and cell-fractionation studies.
Right : 1) A fluorescent antibody technique using 1-dimethylaminonaphthalene 5-sul-phonylchloride and Xylene Red B has been described. The conjugation procedure for each dye with protein is simple without any deterioration of the antibody activity.2) The quality of each conjugate was determined by use of a Beckman spectro-photometer and spectrofluorometer. 3) The viral antigen synthesized in Ehrlich ascites tumor cells infected with ED virus was distinctly visualized by staining of each conjugate, and the specificity of fluorescent staining of antigen was confirmed with the systematic control tests.
XRB-conjugated protein was preferable for the detection of antigen, because it showed brilliant orange fluorescence and could be clearly discriminated from the auto-fluorescene of cells. 4) The viral antigen was detected in the cytoplasm of the infected cells within 6 hous after virus challenge, and increased continuously until a maximum was reached at 24 hours. 5) The observation by mean of fluorescent antibody technique confirms the the previous results obtained by immunochemical and cell-fractionation studies.

Date of correction: April 18, 2008 Reason for correction: - Correction: AFFILIATION Details: Wrong :
1) Institute for Infectious Diseases, University of Tokyo, Tokyo

Right :
1) Institute for Infectious Diseases, University of Tokyo

Date of correction: April 18, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

1) A fluorescent antibody technique using 1-dimethylaminonaphthalene 5-sul-phonylchloride and Xylene Red B has been described. The conjugation procedure for each dye with protein is simple without any deterioration of the antibody activity.2) The quality of each conjugate was determined by use of a Beckman spectro-photometer and spectrofluorometer. 3) The viral antigen synthesized in Ehrlich ascites tumor cells infected with ED virus was distinctly visualized by staining of each conjugate, and the specificity of fluorescent staining of antigen was confirmed with the systematic control tests.
XRB-conjugated protein was preferable for the detection of antigen, because it showed brilliant orange fluorescence and could be clearly discriminated from the auto-fluorescene of cells. 4) The viral antigen was detected in the cytoplasm of the infected cells within 6 hous after virus challenge, and increased continuously until a maximum was reached at 24 hours. 5) The observation by mean of fluorescent antibody technique confirms the the previous results obtained by immunochemical and cell-fractionation studies.

Corresponding author

Correction information

Register with J-STAGE for free!