Abstract
In the cultures containing charcoal, maximum yield of butanol was produced by Cl. butyricum (b) and Cl. pasteurianum (d) after 15 and 22 days of fermentation respectively. The formation of acetone was maximum in the culture of Cl. butyricum (b) after 15 days, and in the culture of Cl. acetobutylicum (f) after 22 days. In the culture containing no charcoal maximum butanol yield was obtained by Cl. acetobutylicum (f), and the yield of acetone was maximum in the culture of Cl. butyricum (a) after 15 days of fermentation. After 22 days, the analysis showed maximum butanol yield in the culture of Cl. pasteurianum (d), and the yield of acetone was maximum in the culture of Cl. butyricum (a).