NTERACTION BETWEEN SALMONELLA E.W ERITIDIS AND TISSUE CULTURED MACROPHAGES DERIVED FROM IMMUNIZED ANIMALS

Abstract

Tissue cultured macrophages derived from guinea pigs and mice immunized with live vaccine or killed vaccine were infected with a virulent strain of Salmonella enteritidis, and the fate of the infected bacteria was pursued. The results obtained are as follows:
1. Tissue cultured macrophages from animals immunized with live vaccine (devoid of specific O antigen) exerted an inhibitory action on intracellular virulent organisms of S. enteritidis regardless of the origin of the serum added to the culture medium.
2. Tissue cultured macrophages from animals immunized with killed vaccine did not exert any inhibitory action on the intracellular virulent S. enteritidis.
3. A slight inhibition of bacterial multiplication in tissue cultured macrophages from mice immunized with killed vaccine was observed only in the presence of immune serum having a high anti-O antibody titre.