EFFECT OF STREPTOMYCIN ON THE INCORPORATION OF S35-SULFATE INTO STREPTOMYCIN-SENSITIVE, STREPTOMYCIN-RESISTANT, AND STREPTOMYCIN-INDIFFERENT CELLS OF A MTCOBACTERIUM

MICHIO TSUKAMURA

doi:10.1111/j.1348-0421.1961.tb00193.x

MICHIO TSUKAMURA

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1961 Volume 5 Issue 2 Pages 133-140

DOI https://doi.org/10.1111/j.1348-0421.1961.tb00193.x

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Published: 1961 Received: November 07, 1960 Available on J-STAGE: April 18, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: April 18, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : Effect of streptomycin on the incorporation of S³⁵-Sulfate and P³²-phosphate into streptomycin-sensitive, streptomycin-resistant, and streptomycin-indifferent cells of Mycobacterium avium, Jucho, were investigated. The streptomycin-resistant strain used was isolated by the multi-step selection with the use of increasing concentrations of streptomycin. Thestreptomycin-indifferent strain used was isolated by the one-step selection in the presence of 1, 000 μg of strepto-mycin per ml. Both strains were resistant to more than 10, 000 μg of streptomycin per ml.
1. Incorporation of S³⁵-sulfate into the protein fraction of streptomycin-sensitive cells was inhibited by the presence of 10 μg of streptomycin hydro-chloride per ml of phopshate buffer solution (pH7.1). However, incorporation of S³⁵-sulfate into acid-soluble fraction was never inhibited by the same concen-tration of the drug.
2. Incorporation of S³⁵-sulfate into protein fractions of streptomycin-resis-tant and streptomycin-indifferent cells was not inhibited by the presence of 10μg of streptomycin per ml, and they, however, were considerably inhibited by the presence of 1, 000μg of streptomycin per ml.
3. Incorporation of S³⁵-sulfate into streptomycin-resistant cells occurred much more slowly than the incorporation of S³⁵-into streptomycin-sensitive cells and streptomycin-resistant cells.
4. Incorporation of P³²-orthophosphate into acid-soluble, nucleic acid and protein fractions of streptomycin-sensitive cells was considerably inhibited by
the presence of 10μg of streptomycin per ml. On the other hand, incorporation of P³² into streptomycin-resistant cells and streptomycin-indifferent cells was not inhibited even by the presence of 1, 000μg of streptomycin per ml.
Right : Effect of streptomycin on the incorporation of S³⁵-Sulfate and P³²-phosphate into streptomycin-sensitive, streptomycin-resistant, and streptomycin-indifferent cells of Mycobacterium avium, Jucho, were investigated. The streptomycin-resistant strain used was isolated by the multi-step selection with the use of increasing concentrations of streptomycin. Thestreptomycin-indifferent strain used was isolated by the one-step selection in the presence of 1, 000 μg of strepto-mycin per ml. Both strains were resistant to more than 10, 000 μg of streptomycin per ml.
1. Incorporation of S³⁵-sulfate into the protein fraction of streptomycin-sensitive cells was inhibited by the presence of 10 μg of streptomycin hydro-chloride per ml of phopshate buffer solution (pH7.1). However, incorporation of S³⁵-sulfate into acid-soluble fraction was never inhibited by the same concen-tration of the drug.
2. Incorporation of S³⁵-sulfate into protein fractions of streptomycin-resis-tant and streptomycin-indifferent cells was not inhibited by the presence of 10μg of streptomycin per ml, and they, however, were considerably inhibited by the presence of 1, 000μg of streptomycin per ml.
3. Incorporation of S³⁵-sulfate into streptomycin-resistant cells occurred much more slowly than the incorporation of S³⁵-into streptomycin-sensitive cells and streptomycin-resistant cells.
4. Incorporation of P³²-orthophosphate into acid-soluble, nucleic acid and protein fractions of streptomycin-sensitive cells was considerably inhibited by the presence of 10μg of streptomycin per ml. On the other hand, incorporation of P³² into streptomycin-resistant cells and streptomycin-indifferent cells was not inhibited even by the presence of 1, 000μg of streptomycin per ml.

Date of correction: April 18, 2008 Reason for correction: - Correction: AFFILIATION Details: Wrong :
1) The Obuso National Sanatorium, Obu, Chita

Right :
1) The Obuso National Sanatorium

Date of correction: April 18, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

Effect of streptomycin on the incorporation of S³⁵-Sulfate and P³²-phosphate into streptomycin-sensitive, streptomycin-resistant, and streptomycin-indifferent cells of Mycobacterium avium, Jucho, were investigated. The streptomycin-resistant strain used was isolated by the multi-step selection with the use of increasing concentrations of streptomycin. Thestreptomycin-indifferent strain used was isolated by the one-step selection in the presence of 1, 000 μg of strepto-mycin per ml. Both strains were resistant to more than 10, 000 μg of streptomycin per ml.
1. Incorporation of S³⁵-sulfate into the protein fraction of streptomycin-sensitive cells was inhibited by the presence of 10 μg of streptomycin hydro-chloride per ml of phopshate buffer solution (pH7.1). However, incorporation of S³⁵-sulfate into acid-soluble fraction was never inhibited by the same concen-tration of the drug.
2. Incorporation of S³⁵-sulfate into protein fractions of streptomycin-resis-tant and streptomycin-indifferent cells was not inhibited by the presence of 10μg of streptomycin per ml, and they, however, were considerably inhibited by the presence of 1, 000μg of streptomycin per ml.
3. Incorporation of S³⁵-sulfate into streptomycin-resistant cells occurred much more slowly than the incorporation of S³⁵-into streptomycin-sensitive cells and streptomycin-resistant cells.
4. Incorporation of P³²-orthophosphate into acid-soluble, nucleic acid and protein fractions of streptomycin-sensitive cells was considerably inhibited by the presence of 10μg of streptomycin per ml. On the other hand, incorporation of P³² into streptomycin-resistant cells and streptomycin-indifferent cells was not inhibited even by the presence of 1, 000μg of streptomycin per ml.

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