Abstract
1. The purified endotoxin, a lipopolysaccharide-protein complex, was adsorbed to Amberltic IRC 50, XE 64 and Dowex 50 at acidic pH and eluted successfully at alkaline pH. This complex can be subjected to column chromatography using Amberlite XE 64 without the loss of its chemical and biological properties.
Though there was 100 per cent adsorption of the endotoxin on the basic resins, attempts to elute the endotoxin resulted in a failure.
2. The purification and chromatography of Pseudomonas aeruginosa phage particles using Atnberlite IRA 410 could be achived Successfully without the loss of their activities. They could also easily be separated frtm the high molecular weight antigenic substances such as the endotoxin.
