CHOPPED MEAT BROTH AS A MEDIUM FOR THE PRODUCTION OF TOXINS BY CLOSTRIDIA

I. PRODUCTION OF TOXINS BY CLOSTRIDIUM WELCHII

Abstract

1. Inconsistent amounts of alpha-toxin produced in chopped meat broth were demonstrated to be due to some agents released from meat particles when they were autoclaved at alkaline pH. This substance was shown to consist mainly of fatty acids. On the contrary the fat fraction showed no effect on the production of alpha-toxin.
2. The capacity to produce alpha-toxin in a chopped meat broth was improved to give 11Lb/ml.