1963 Volume 7 Issue 3 Pages 87-95
1) Four strains of Pseudomonas aeruginosa, Nos. 134, 145, 117 and 58, with different pigment producing ability on glycerol-agar medium, were cultivated on glutamic acid medium to observe their mode of pigment production.
2) The glutamic acid medium developed by the authors is of a simplecomposition, and the production of pigments and fluorescent substances in this medium is so rapid and brilliant that it is valuable in the study of pigment production by the Pseudomonas.
3) Four strains of Ps. aeruginosa were grown in the glutamic acid medium, and gel filtration was carried out on their culture filtrate. The following results were obtained.
a) The fluorescent material produced by Ps. aeruginosa can be separated into at least two portions on the dextran gel column. They were designated as Fluorescence I and Fluorescence II.
b) It is not clear whether the greenish yellow part of Fluorescence I appearing in the early stage of cultivation of strains No. 134 and 145 is or not identical to Fluorescence I.
c) The red and orange pigments of strain No. 145 and the blue pigment of No. 58 are believed to be of different substances.
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