Abstract
The effect of heterologous antiserum and complement on glucose metabolism of rat ascites tumor cells was examined to clarify the mechanism of the cytotoxic effect of antibody.
1. Lactate-production of the target cells was inhibited by the cooperation of antiserum and complement, while no inhibition was observed when the cells were subjected to either antiserum or complement alone. The inhibition of lactateproduction was observed under both aerobic and anaerobic conditions. No effect of the antiserum and complement was observed on the oxygen consumption of the cells.
2. The amount of antiserum necessary for the inhibition of lactate-production of the cells was determined in the presence of a definite amount of complement, and it was found that the degree of inhibition was not in parallel with the concentration of antiserum beyond a certain threshold. On the other hand, a parallelism was observed between the amount of complement and the rate of inhibition of lactateproduction when different amounts of complement were added to a definite amount of antiserum.
3. Similar inhibitory effects of the antiserum and complement on lactateproduction of the cells from glucose, fructose-1, 6-diphosphate, and pyruvate suggested that the inhibition occurred toward the final step of glycolytic process of the cells.
