MICROBIOLOGY and IMMUNOLOGY
Online ISSN : 1348-0421
Print ISSN : 0385-5600
ISSN-L : 0385-5600
Some Physicochemical Characteristics of an Immune Lymphocyte Product Which Inhibits the Multiplication of Toxoplasma within Mouse Macrophages
Toshikazu SHIRAHATAKiheiji SHIMIZU
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1979 Volume 23 Issue 1 Pages 17-30

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Abstract
In response to antigenic stimulation, the splenic lymphocytes from Toxoplasma-infected mice produce a factor which is called by the authors the Toxoplasma growth inhibitory factor (Toxo-GIF) and which inhibits the multiplication of Toxoplasma within nonimmune macrophages in vitro. In this study, partial characterization of murine Toxo-GIF was examined using Sephadex G-100 gel-filtration, isoelectric focusing, zonal electrophoresis and heat and enzymatic treatment.
Peak activity of Toxo-GIF was found in a Sephadex G-100 fraction with a similar molecular size to that of the ovalbumin. The molecular weight of Toxo-GIF was calculated to be between 38, 000 and 55, 000. Toxo-GIF was stable to heating at 56 C for 30 min but lost its activity at 80 C for 30 min or by exposure to pH values of 5 and 2. Exposure of Toxo-GIF to water-insoluble chymotrypsin or neuraminidase markedly decreased its ability to induce enhanced microbicidal activity of cultured macrophages, suggesting that Toxo-GIF was a glycoprotein. Furthermore, Toxo-GIF migrated in a region cathodal to mouse albumin on agar zone electrophoresis. Isoelectric focusing of active Sephadex fractions showed a well-defined peak of Toxo-GIF activity with an isoelectric point of pH 4.9 to 5.9.
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