Abstract
Adenovirus type 12 (Ad12) -transformed mouse cells were examined for their susceptibility to cell-mediated immunity in vitro, with respect to the activity of the virus-specific surface (S) antigen in the cells. A transformed cell line, C57ATl, was established from embryonic cells of C57BL/6 mice by Ad12 infection. In fluorescent antibody tests, the transformed cells were positive for the S antigen when the cells were maintained as cultures, whereas when the cells were grown as tumors in animals they became negative for the antigen (referred to as S (+) and S (-) cells, respectively). These S (+) and S (-) cells were subjected to the 51Cr-release test for cell lysis by immune spleen cells (ISC) raised in syngeneic mice by Ad12 infection. When the S (+) cells at various passage levels were exposed to ISC, all of them were lysed extensively and to a similar extent irrespective of their passage history. In contrast, the S (-) cells were consistently refractory to the action of ISC. In addition, the cytotoxic action of ISC was markedly impeded by pretreating the S (+) cells with antiserum to the S antigen, or the ISC with anti-Thy-1, 2 serum plus complement. Taken these findings together, the S (+) cells were assumed to be injured by ISC through direct interaction of the S antigen with T-lymphocytes.
