4-Hydroxy-3-Nitrophenyl (NP) Acetyl-Hapten Specific Lymphocyte Proliferation

I. Mice Bearing Igh-1^b Allotype Can Cross-React with 4-Hydroxy-5-Iodo-3-Nitrophenyl (NIP) Acetyl Hapten

Abstract

Hapten specific T cell proliferation was induced in several strains of mice. When lymph node T cells from 4-hydroxy-3-nitrophenyl acetyl-keyhole lympet hemocyanin (NP-KLH)-primed mice were stimulated in vitro with NP-polymer glutamic acid-lysine-phenyl alanine (NP-GLØ) or NP-ovalbumin (NP-OVA), they displayed a good level of proliferative responses. It was observed that NP-GLØ could induce NP-hapten specific proliferation even with NP-KLH lymphocytes from GLØ non-responder strains.
NP-KLH primed lymphocytes from C57BL/6 (H-2^b, Igh-1^b), CKB (H-2^k, Igh-1^b), CWB (H-2^b, Igh-1^b), and B10.BR (H-2^k, Igh-1^b) mice showed good proliferative responses to both 4-hydroxy-5-iodo-3-nitrophenyl (NIP) acetyl-GLØ and NIP-OVA antigens. However, NP-KLH primed lymphocytes from C3H/He (H-2^k, Igh-1^j) and C3H. SW (H-2^b, Igh-1^j) mice displayed poor proliferative responses to NIP-GLØ and NIP-OVA antigen. These results suggested that the gene coding for the NIP-cross-reaction might be mapped in the Ig heavy-chain linked locus.