Efficient Adsorption of Lysostaphin on Bacterial Cells of Lysostaphin-Resistant Staphylococcus aureus Mutant

Junji Sakurada; Miyo Murai; Zhijun Li; Akemi Usui; Keiko Seki; Kiyoshi Kobayashi; Yoshiko Sumi; Hiroko Jitsukawa; Shogo Masuda

Efficient Adsorption of Lysostaphin on Bacterial Cells of Lysostaphin-Resistant Staphylococcus aureus Mutant

Junji Sakurada, Miyo Murai, Zhijun Li, Akemi Usui, Keiko Seki, Kiyoshi Kobayashi, Yoshiko Sumi, Hiroko Jitsukawa, Shogo Masuda

Author information

Junji Sakurada
Department of Bacteriology, The Jikei University School of Medicine
Miyo Murai
Department of Bacteriology, The Jikei University School of Medicine
Zhijun Li
Department of Bacteriology, The Jikei University School of Medicine
Akemi Usui
Department of Bacteriology, The Jikei University School of Medicine
Keiko Seki
Department of Bacteriology, The Jikei University School of Medicine
Kiyoshi Kobayashi
Department of Bacteriology, The Jikei University School of Medicine
Yoshiko Sumi
Department of Bacteriology, The Jikei University School of Medicine
Hiroko Jitsukawa
Department of Bacteriology, The Jikei University School of Medicine
Shogo Masuda
Department of Bacteriology, The Jikei University School of Medicine

Keywords: Staphylolytic endopeptidase, Lysostaphin, Staphylococcus simulans biovar staphylolyticus

JOURNAL FREE ACCESS

1993 Volume 37 Issue 1 Pages 29-34

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Abstract

A simple and efficient method for the purification of staphylolytic endopeptidase (lysostaphin) contained in culture supernatant of Staphylococcus simulans biovar staphylolyticus strain by adsorption of the enzyme on bacterial cells of lysostaphin-resistant S. aureus mutant was successfully devised. Lysostaphin was sufficiently adsorbed on the heat-killed mutant cells derived from S. aureus Cowan I and efficiently eluted by 3M KSCN. Enzyme preparation obtained by a single procedure of the affinity purification was pure enough for practical use. The yield of the enzyme was 25mg from 1 liter culture and recovery rate was 64%.

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