1995 Volume 39 Issue 1 Pages 81-86
To evaluate the relationship between the sugar chain structure and biological activity, fibroblast-derived glycosylated human interferon-β, Chinese hamster ovary cell-derived glycosylated recombinant human interferon-β and Escherichia coli-derived unglycosylated recombinant human interferon-β were evaluated using human hepatoblastoma cells in vitro. Native fibroblast interferon-β expressed more cell-growth inhibitory action, 2'5'-oligoadenylate synthetase induction, and the inhibition of hepatitis B virus DNA replication than its asialoform and two recombinant interferon-βs. These results showed that the sugar chain structure of human interferon-β affects its biological activity on human hepatoblastoma cells.